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BioChain Institute ishyb in situ hybridization kit
Ishyb In Situ Hybridization Kit, supplied by BioChain Institute, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ishyb+in+situ+hybridization+ish+kit/IsHyb+In+Situ+Hybridization+(ISH)+Kit+for+20+slides/pmc11065892-359-0-5
Average 93 stars, based on 1 article reviews
ishyb in situ hybridization kit - by Bioz Stars, 2026-09
93/100 stars

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In Situ Hybridization:

Article Title: Expression of the RNA-stabilizing protein HuR in ischemia-reperfusion injury of rat kidney
Article Snippet: .. In situ hybridization was performed on 5-μm sections using an IsHyb In Situ Hybridization (ISH) Kit (BioChain Institute, Hayward, CA) according to instructions from the manufacturer with modifications. ..

Article Title: In situ hybridization detection of microRNAs
Article Snippet: .. IsHyb In Situ Hybridization (ISH) Kit (Biochain). ..

Article Title: Sur1-Trpm4 Cation Channel Expression in Human Cerebral Infarcts
Article Snippet: Digoxigenin labeled probes, antisense (5′- CCGAGAGTGGAATTCCCGGATGAGGCGGTAACGCTGC-3′) and sense (5′-GCAGCGTTACCGCCTCATCCGGGAATTCCACTCTCGG-3′), designed to hybridize to nucleotides 3288–3324 located within coding sequence of the human Trpm4 gene ( {"type":"entrez-nucleotide","attrs":{"text":"NM_017636","term_id":"304766649","term_text":"NM_017636"}} NM_017636 ), were supplied by IDT (Integrated DNA Technologies, Inc., Coralville, IA). .. In situ hybridization was performed on 10-μm sections of acute lesions (cases 1-4) and controls using an IsHyb In Situ Hybridization (ISH) Kit (Biochain Institute, Inc., Newark, CA) according to the manufacturer's protocol. ..

Article Title: Tumor suppressor microRNA-493 decreases cell motility and migration ability in human bladder cancer cells by down-regulating RhoC and FZD4
Article Snippet: In situ hybridization Tissue arrays were purchased from US Biomax, inc. (Rockville, MD, USA). .. For in situ hybridization, IsHyb In Situ Hybridization (ISH) Kit was used according to the manufacturer’s instructions (BioChain, Hayward, CA, USA). .. A tissue array slide was incubated overnight with a 1:500 dilution of miRCURY LNA Detection probe 5′-DIG labeled for miR-493 (EXIQON, Woburn, MA, USA) and overnight at 4°C with a 1:200 dilution of AP-conjugated anti-digoxingenin antibody.

Article Title: A Novel Saliva-Based miRNA Profile to Diagnose and Predict Oral Cancer
Article Snippet: .. 4.5.3 miRNA in-situ hybridization Unstained and hematoxylin and eosin (H&E) stained Formalin Fixed Para n Embedded (FFPE) tissue slides were obtained from the RBWH, and miRNA in-situ hybridization was performed using IsHyb In Situ Hybridization (ISH) Kit following the manufacturer’s instructions (Biochain, San Francisco, California) and as previously reported 49. .. Proteinase K was purchased from Qiagen, MD, USA. hsa-miR-7-5p miRCURY LNA miRNA Detection probe (double DIG (Digoxigenin) labelled) (Qiagen) or negative control miRNA scrambled probe (double DIG labelled) (Integrated DNA technologies) or u6 snRNA positive control probe (double DIG labelled) were used at 50nM per slide.

Staining:

Article Title: A Novel Saliva-Based miRNA Profile to Diagnose and Predict Oral Cancer
Article Snippet: .. 4.5.3 miRNA in-situ hybridization Unstained and hematoxylin and eosin (H&E) stained Formalin Fixed Para n Embedded (FFPE) tissue slides were obtained from the RBWH, and miRNA in-situ hybridization was performed using IsHyb In Situ Hybridization (ISH) Kit following the manufacturer’s instructions (Biochain, San Francisco, California) and as previously reported 49. .. Proteinase K was purchased from Qiagen, MD, USA. hsa-miR-7-5p miRCURY LNA miRNA Detection probe (double DIG (Digoxigenin) labelled) (Qiagen) or negative control miRNA scrambled probe (double DIG labelled) (Integrated DNA technologies) or u6 snRNA positive control probe (double DIG labelled) were used at 50nM per slide.

Formalin-fixed Paraffin-Embedded:

Article Title: A Novel Saliva-Based miRNA Profile to Diagnose and Predict Oral Cancer
Article Snippet: .. 4.5.3 miRNA in-situ hybridization Unstained and hematoxylin and eosin (H&E) stained Formalin Fixed Para n Embedded (FFPE) tissue slides were obtained from the RBWH, and miRNA in-situ hybridization was performed using IsHyb In Situ Hybridization (ISH) Kit following the manufacturer’s instructions (Biochain, San Francisco, California) and as previously reported 49. .. Proteinase K was purchased from Qiagen, MD, USA. hsa-miR-7-5p miRCURY LNA miRNA Detection probe (double DIG (Digoxigenin) labelled) (Qiagen) or negative control miRNA scrambled probe (double DIG labelled) (Integrated DNA technologies) or u6 snRNA positive control probe (double DIG labelled) were used at 50nM per slide.



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